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. 2019 Sep 30;7:e7716. doi: 10.7717/peerj.7716

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©2019 Cheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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M: protein marker; lane 1: CK (without IPTG); lane 2: whole-cell lysate after pET32a-Hir induction; lane 3: supernatant after pET32a-Hir induction; lane 4: precipitate after pET32a-Hir induction; lane 5: eluted Hir fusion protein; lane 6: fusion protein digested by enterokinase; lane 7: purified Hir.