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. 2019 Sep 30;7:e7766. doi: 10.7717/peerj.7766

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© 2019 Yan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Amplification curves of qPCR for detection of wheat loose smut in field by primer Y-430. (B) Melting curves of qPCR for detection of wheat loose smut in field by primer Y-430. (C) LAMP detection of wheat loose smut in the field under natural light. Tube 1–2: the results of LAMP assay with L-139. Tube 3–4: the results of LAMP assay with L-988. (D) Detection by agarose electrophoresis after LAMP assay. Strip 1–2: the results of LAMP assay with L-139. Strip 3–4: the results of LAMP assay with L-988.