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. 2019 Sep 30;7:e7766. doi: 10.7717/peerj.7766

Figure 5. Temperature optimization of qPCR.

Figure 5

(A) Amplification curves of qPCR for U. tritici under temperature gradients. 1–7: 52, 54, 56, 58, 60, 62, 64 °C. (B) Gel electrophoresis of qPCR on U. tritici under temperature gradients M: Maker, 1–7: 52, 54, 56, 58, 60, 62, 64 °C.