Fig. 3.

Metabolic pathways identified in atribacterial MAGs and SAGs with contamination less than 8%. Newly assembled and co-assembled MAGs were indicated in bold, and MAGs that contain fumarate addition enzymes were highlighted in red. Genome with completeness >90% that meet the rank of high-quality were underlined [26]. The colour code of the columns and dots in the igure are the same to the colour code of stars in Figs. 1a,  2b and the enzyme names were colored and grouped based on their functions. Pathways that have been previously described in Atribacteria lineages were considered as present when 75% of the genes involved in the pathway were detected. Pathways that have not been found in Atribacteria lineages before were defined as present only if all genes involved in the pathway were detected. Identification of the pathways was carried out with pathway mapping on both RAST server and KEGG server. Results were manually checked by BLASTP of the genes against NCBI-nr database. Isolated microorganisms were known to be capable of anaerobic fumarate addition to hydrocarbons were used as positive controls for identification of genes involved in anaerobic hydrocarbon degradation. Abbreviations are: bssABC, benzylsuccinate synthase; nmsABC, naphthyl-2-methyl-succinate synthase; assABC alkylsuccinate synthase; bbsEF, benzylsuccinate-CoA transferase; bbG, benzylsuccinyl-CoA dehydrogenase; bbsH, enoyl-CoA hydratase; bbsCD, 3-hydroxyacyl-CoA dehydrogenase; bbsAB; 3-oxoacyl-CoA thiolase; bnsEF, naphthyl-2-methyl-succinate-CoA transferase; bnsG, naphthyl-2-methyl-succinyl-CoA dehydrogenase; bnsH, naphthyl-2-methylene-succinyl-CoA hydratase; bnsCD, naphthyl-2-hydroxymethyl- succinyl-CoA dehydrogenase; bnsAB, naphthyl-2-oxomethyl-succinyl-CoA thiolase; badA, benzoate-CoA ligase; hbaA, 4-hydroxybenzoate-CoA ligase; bcrA-D; acs, acetyl-CoA synthetase; pka, phosphate acetyltransferase; ack, acetate kinase. A detail information of the genes in this figure could be found in Supplemenatary Table S5