Table 2.
Primer information for TCS genes
| Gene | Forward primer (5′→3′) | Reverse primer (5′→3′) | Amplified region of the registered sequence | |
|---|---|---|---|---|
| TCS1 | ACAGCCAGCGCTAGAAAATG | CATGGCATGGTCAATGAAAA | 4509–5036 bp: part of 2nd exon and 2nd intron | |
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| TCS2 | 1st PCR | TTTGTTCAAATGAATCGAAATGA | CAAGTCCGCTGCGTTAAGAG | |
| 2nd PCR | AAATAATGAAATGTGTGAACCAAA | CACTGGCATTGTCATTGAGG | 3029–3884 bp: part of 1st intron and 2nd exon | |
|
| ||||
| TCS3 | CATGAACAGAGGAGAAGGAGAAA | ATAGGGTGCCAACGTGACAT | 425–1251 bp: part of putative 1st exon and 1st intron | |
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| TCS4 | AATGCTAGTGGATGGGTTGG | CATGGCATGGTCAATGAAAA | 3293–4230 bp: part of 1st intron, 2nd exon and part of 2nd intron | |
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| TCS5 | 1st PCR | CATGAACAGAGGAGAAGGAGAAA | CAACTGCATTTTCTAGCGCTGGC | |
| 2nd PCR | CATGAACAGAGGAGAAGGAGAAA | CCCAACCATCCACTAGCATT | 569–1419 bp: part of 1st exon and 1st intron | |
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| ||||
| TCS6 | CATGAACAGAGGAGAAGGAGAAA | AGTTCCAGTGTTTGGCAATTC | 608–3248 bp: part of 1st exon, 1st intron and part of 2nd exon | |
GenBank Accession Nos of TCS1–TCS6 are JX647690–JX647695.