Table 1.
Primers used in quantitative RT-PCR
| Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| 18s | GAATTCCCAGTAAGTGCGGGTCATA | CGAGGGCCTCACTAAACCATC |
| Acan | CTAGCTGCTTAGCAGGGATAACG | GATGACCCGCAGAGTCACAAAG |
| Sox9 | AGGAAGCTGGCAGACCAGTACC | GGGTCTCTTCTCGCTCTCGTTCA |
| Col2a1 | CTGGTGGAGCAGCAAGAGC | GTGGACAGTAGACGGAGGAAAG |
| Col1a1 | ACCTCCGGCTCCTGCTCCTCTTA | GACAGCACTCGCCCTCCCGTTTTT |
| Krt19 | CCGACCTGGAGATGCAGATAGAG | TAGGTCAATGCCTGGTGTGGAA |
| Pax1 | CTCCTTAGGGCCACCGCT | TTAATTGACGTTCCGATCCTGC |
| Gdf10 | ACAGTGTGCAGAGGGACAGG | CTTCTGGTCGATCACATCCAGC |
| Cd24 | CCGAGCATCCAGAGAGTCG | GCAATAAATCTGCGTGGGTAGG |
| Shh | CTATGAGGGTCGAGCAGTGG | CGGAGTTCTCTGCTTTCACAG |
| Gli1 | CTATGGTGAGCCATGCTGTC | GAAAGTCCTTCTGTTCCCATGC |
| Gli2 | CCCCTACCGATTGACATGCG | GAAAGCCGGATCAAGGAGATG |
| Ptch1 | GGACTTACGTGGAGGTGGTT | TGGTTAAACAGGCGTAGGCA |