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. 2019 Sep 26;55(5):1033–1048. doi: 10.3892/ijo.2019.4886

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Copyright: © Ferreira et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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CEA from NSCLC associates with sLe^x/sLe^a and has reactivity with E-selectin. (A) Western blot analysis of tumour lysates from CEA-positive NSCLC tissues. CEA-positive NSCLC tissues were resolved via SDS-PAGE and immunoblotted with HECA-452 mAb, E-Ig and anti-CEA mAb. (B) Western blot analysis of immunoprecipitated CEA. CEA was immunoprecipitated from the tumour lysate of patient 7, and then resolved via SDS-PAGE and blotted. Left, HECA-452 staining; middle, E-Ig staining; right, anti-CEA mAb staining. CEA, carcinoembryonic antigen; E-Ig, mouse E-selectin-human Fc Ig chimera; HECA, HECA-452; IP, immunoprecipitation; mAb, monoclonal antibody; NSCLC, non-small cell lung cancer; sLe^a/x, sialyl Lewis a/x.