Table 5.
Mean fluorescence intensity (MFI) of expression of cluster of differentiation (CD) 11b, and 62L, by peripheral blood neutrophils of newly weaned heifers injected repeatedly with saline (CTL−), increasing doses of LPS over a 10-d period (CTL+), or CTL+ plus fed the diet supplemented with a low (OBE-L; 0.04% of diet DM) or a high (OBE-H; 0.16% of diet DM) dose of an olive oil bioactive extract (OBE)
Treatments1 | Contrasts P-value | |||||||
---|---|---|---|---|---|---|---|---|
Item2 | CTL− | CTL+ | OBE-L | OBE-H | SEM | CTL+ vs. OBE | OBE-L vs. OBE-H | CTL− vs. CTL+ |
Day 0 | ||||||||
Neutrophil | ||||||||
CD11b (MFI × 1,000) | 1.9 | 2.1 | 1.8 | 1.5 | 0.18 | 0.03 | 0.14 | 0.33 |
CD62L (MFI × 1,000) | 0.63 | 0.57 | 0.72 | 0.60 | 0.08 | 0.38 | 0.28 | 0.63 |
Day 10 | ||||||||
Neutrophil | ||||||||
CD11b (MFI × 1,000) | 1.7 | 2.2 | 2.0 | 2.0 | 0.43 | 0.30 | 0.70 | 0.03 |
CD62L (MFI × 1,000) | 0.53 | 0.62 | 0.60 | 0.74 | 0.11 | 0.38 | 0.05 | 0.22 |
1CTL− = control diet + saline injections; CTL+ = control diet + LPS injections; OBE-L = control diet + olive oil bioactive extract at 0.04% of the diet + LPS injection; OBE-H = control diet + olive oil bioactive extract at 0.16% of the diet + LPS injection.
2Results are expressed as least square means from 12 heifers per treatment.