Table 2.
The sequences of primers
| Gene1 | Sequence number | Product length, bp | Primer sequences, 5′→3′ | T m value |
|---|---|---|---|---|
| ZO-1 | XM_003353439.1 | 169 | F: GAGGATGGTCACACCGTGGT R: GGAGGATGCTGTTGTCTCGG |
60 ℃ |
| Occludin | NM_001163647.2 | 105 | F: TGGGTTAAAAACGTGTCGGC R: CACTTTCCCGTTGGACGAGT |
60 ℃ |
| Claudin-1 | NM_001161635.1 | 155 | F: ACCCCAGTCAATGCCAGATA R: GGCGAAGGTTTTGGATAGG |
58 ℃ |
| TLR4 | AB232527 | 113 | F: CAGATAAGCGAGGCCGTCATT R: TTGCAGCCCACAAAAAGCA |
55 ℃ |
| MγD88 | AB292176.1 | 148 | F: GATGGTAGCGGTTGTCTCTGAT R: GATGCTGGGGAACTCTTTCTTC |
60 ℃ |
| NF-κB p65 | EU399817.1 | 133 | F: CAGCCCTATCCCTTTACG R: GCCACAGCCTGAGCAA |
60 ℃ |
| TNF-α | NM_214022.1 | 168 | F: CCACGCTCTTCTGCCTACTGC R: GCTGTCCCTCGGCTTTGAC |
61 ℃ |
| IL-6 | NM_9405217033 | 146 | F: TCAGTCCAGTCGCCTTCT R: CCTTTGGCATCTTCTTCC |
56 ℃ |
| IL-10 | NM_214041.1 | 136 | F: CACTGCTCTATTGCCTGATCTTCC R: AAACTCTTCACTGGGCCGAAG |
56 ℃ |
| IL-1β | NM_9405217038 | 165 | F: CAAGGAAGTGATGGCTAA R: ACCAAGGTCCAGGTTTT |
54 ℃ |
| GAPDH | AF017079.1 | 178 | F: ACATCAAGAAGGTGGTGAAG R: ATTGTCGTACCAGGAAATGAG |
60 ℃ |
1ZO-1, zonula occludens-1; TLR4, toll-like receptor 4; MγD88, myeloid differentiation factor 88; NF-κB p65, nuclear factor-kappa B p65; TNF-α, tumor necrosis factor; IL-6, interleukin-6; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.