Table 2.
Pairs of primers used for quantitative real-time PCR assay1
| Gene | Primer sequence (5′-3′) | Accession NO. |
|---|---|---|
| β-actin | F:ATGCTTCTAGGCGGACTGT | AY550069 |
| R:CCATCCAACCGACTGCT | ||
| FXR | F:AAGGACCGAGAGGCAGTAGAGAAG | NM_001287412.1 |
| R:TCTGCGTGGTGATGGTTGAATGTC | ||
| CYP7A1 | F:GAAAGAGAGACCACATCTCGG | NM_001005352.3 |
| R:GAATGGTGTTGGCTTGCGAT | ||
| CYP27A1 | F:ACTGAAGACCGCGATGAAAC | NM_001243304.1 |
| R:CAAAGGCGAATCAGGAAGGG | ||
| CYP8B1 | F:TTCCGCAAGTTCGACCGCATG | NM_214426.1 |
| R:GCTGCTTATGCCGTGCCTCTC | ||
| BSEP | F:GGAGCAAGAGCCAGTTCTGTTCTC | GQ169125.1 |
| R:CCTTCTTGGCAGCGTGGATGAC | ||
| MRP2 | F:TCCAACAGGTGGCTTGCAGTTC | AF403247.1 |
| R:GTCATCCTCACCAGCCAGTTCAG | ||
| SLC10A1 | F:TGACCACCTGCTCCACCTTCC | XM_001927695.5 |
| R:AGAGCACTGTGACAGCAATAGCG | ||
| SLCO1A2 | F:ATGTGGTGAGTCAGGGGCAT | NM_001256595.1 |
| R:CCTCTTAGTGCTGCTGGCAA |
1FXR = farnesoid X receptor; CYP7A1 = cholesterol 7α-hydroxylase; CYP27A1 = sterol 27-hydroxylase; CYP8B1 = sterol 12α-Hydroxylase; BSEP = bile acid response element; MRP2 = multidrug resistance associated protein 2; SLC10A1 = solute carrier family 10 member 1; SLCO1A2 = solute carrier organic anion transporter family member 1A2.