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Attachment of azide as well as alkyne handles onto heat shock protein 27 (A, B), which can be employed for click derivatization to introduce labels (biotin). HPLC traces (214 nm) of substrate and crude reaction mixtures (after 2 h) of the Hsp27 modifications (C) and mass spectra of the purified Hsp27 with bioconjugation handles and crude CuAAC product are depicted (D).
