Table 1. Mutant reference standard input was established using ddPCR.

Replicates are increased at the lowest concentrations to account for stochastic effects.

Name	Mutant Input	WT input (human placental DNA)	Replicates per Standard
Standard A	~5120 copies	~14,000 copies	3
Standard B	~640 copies	~14,000 copies	3
Standard C	~80 copies	~14,000 copies	3
Standard D	~10 copies	~14,000 copies	3
Standard E	1:2 of Std D	~14,000 copies	20
Standard F	1:2 of Std E	~14,000 copies	20
Standard G	1:2 of Std F	~14,000 copies	20