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Affinity and specificity analysis of αp38mb by ITC. ITC experiments are reported for the titration of αp38mb into solutions of 12.5 μM p38α (a), 13 μM ERK2 (b), and 15 μM JNK1 (c). The binding affinity (KD) of αp38mb for p38α was measured to be 649±100 nM, whereas no specific binding was detected of αp38mb to either ERK2 or JNK1.
