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. Author manuscript; available in PMC: 2019 Oct 3.
Published in final edited form as: Biochemistry. 2018 Jul 19;57(30):4526–4535. doi: 10.1021/acs.biochem.8b00408

Figure 6:

Figure 6:

The binding interaction between the engineered monobody and p38α is retained in cell culture. (a) Monobodies were co-expressed with p38α in cells. Wildtype monobody (WTmb) or engineered monobody (αp38mb) containing the HA-epitope tag was transiently co-transfected with V5-tagged p38α in HEK293 cells. Immunoblotting shows co-expression of p38α with either WTmb or αp38mb. (b) Constructs from (a) were used in a pull-down assay where p38α was immunoprecipitated with anti-V5 antibody followed by Immunoblotting analysis of V5 and HA-epitope tags. Co-immunoprecipitation of αp38mb with p38α was observed, but WTmb was not co-immunoprecipitated.