Table 1.

Sequence alignment of the BC and FG loops of monobody. ‘X’ represents positions completely randomized in library design. Mutations differing between G4mb and αp38mb are highlighted green. Position 29a refers to the extension of the BC loop for library design and subsequent mutants isolated from library screening.

Monobody	BC loop	FG loop
	22 . . .26 . . .29a30	75 . . . .80 . . . .85 . .88
Wild type monobody	W D A P A V T V - R	V T G R G D S P A S S K P I
674mb	W V P P W R P V - R	V N G I G P Q L T I P D G I
Monobody Library	W D X X X X X X X R	V T G X P G L K I P X K P I
C6mb	W D S P R W W W V R	V T G M P G L K I P L K P I
G4mb	W D P P R C R R I R	V T G M P G L K I P R K P I
αp38mb	W D P P R C R R A R	V T S M P R L G I P R K S I