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. 2019 Mar 9;10(10):726–744. doi: 10.1007/s13238-019-0614-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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AKT1, but not its family members, AKT2 or AKT3, regulates ciliogenesis and disassembly. (A–D) Effect of AKT family members: BEAS2B cells were transfected with scrambled control siRNA, siAKT1, siAKT2 or siAKT3, then treated with DMSO or MLN4924 (0.3 μmol/L) under cilia initiation condition for indicated periods of time, followed by western blotting with indicated Abs (A) or immunofluoresence staining of cilia and photography (B). Percentage of ciliated cells were counted (C) or cilia length measured (D), the results plotted. Shown is mean ± SEM from three independent experiments. **P < 0.01, ***P < 0.001. (E–H) Effect of knockdown of AKT family members on cilia disassembly and growth: BEAS2B cells were transfected with scrambled control siRNA, siAKT1, siAKT2 or siAKT3, then treated with DMSO or MLN4924 (0.3 μmol/L) under cilia disassembly condition for 2 h, followed by Western blotting with indicated Abs (E) or immunofluoresence staining of cilia and photography (F). Percentage of ciliated cells were counted (G) or cilia length measured (H), the results plotted. Shown is mean ± SEM from three independent experiments. **P < 0.01

AKT1, but not its family members, AKT2 or AKT3, regulates ciliogenesis and disassembly. (A–D) Effect of AKT family members: BEAS2B cells were transfected with scrambled control siRNA, siAKT1, siAKT2 or siAKT3, then treated with DMSO or MLN4924 (0.3 μmol/L) under cilia initiation condition for indicated periods of time, followed by western blotting with indicated Abs (A) or immunofluoresence staining of cilia and photography (B). Percentage of ciliated cells were counted (C) or cilia length measured (D), the results plotted. Shown is mean ± SEM from three independent experiments. **P < 0.01, ***P < 0.001. (E–H) Effect of knockdown of AKT family members on cilia disassembly and growth: BEAS2B cells were transfected with scrambled control siRNA, siAKT1, siAKT2 or siAKT3, then treated with DMSO or MLN4924 (0.3 μmol/L) under cilia disassembly condition for 2 h, followed by Western blotting with indicated Abs (E) or immunofluoresence staining of cilia and photography (F). Percentage of ciliated cells were counted (G) or cilia length measured (H), the results plotted. Shown is mean ± SEM from three independent experiments. **P < 0.01