Figure 6.

Cross-talk between AKT1 and pVHL in regulation of ciliogensis. (A–D) Effect of VHL on AKT level, activity, and ciliogenesis: BEAS2B cells were transfected with vector, HA-AKT1 or VHL alone or in combination, followed by serum starvation for indicated time points, and Western blotting with indicated Abs (A), or immunofluoresence staining of cilia and photography (B). Percentage of ciliated cells were counted (C) or cilia length measured (D), and the results plotted. Shown is mean ± SEM from three independent experiments. **P < 0.01, ***P < 0.001. (E–H) Effect of pVHL knockdown on ciliogenesis triggered by AKT1 knockdown: BEAS2B cells were transfected with scrambled control siRNA, siAKT1, or siVHL alone or in combination, followed by serum starved for 6 h, and Western blotting with indicated Abs (E) or immunofluoresence staining of cilia and photography (F). Percentage of ciliated cells were counted (G) or cilia length measured (H), and the results plotted. Shown is mean ± SEM from three independent experiments. ***P < 0.001