Figure 7.

MLN4924 inhibits hair regrowth by blocking ciliogenesis in mouse skin. (A) The dorsal hairs of mice were removed on day 1. On day 2, mice were injected with MLN4924 (30 mg/kg) along with vehicle control, once a day, 5 days per week for 2 weeks. The photo images were taken before and after 2 weeks of treatment. (B and C) Mice skin were harvested at the end of 2-week treatment, fixed, embedded, sectioned, stained with H&E and photographed. The hair follicles were counted and result blotted (B), or skin sections were stained with immonofluorescence using ciliary marker acetylated tubulin (red), positive cells were counted. All scale bars are 200 µm. Shown is mean ± SEM from randomly selected 5 skin areas harvested from at least two mice. (D) The working model. See text for details