Fig. 7. RAS status modulates cell sensitivity during lysosomal pH alkalinization induced by VP.

a Representative immunoblots and quantification, b for pERK, tERK, pan RAS and β-actin in HuH7 and HepG2 cells after VP (20 μM, 24 h), SF (5 μM, 24 h) treatment and both compounds combined together. c Mean of relative viability and d LDH release of a panel of eight different HCC cell lines was analyzed after 72 h of verteporfin (VP – 20 μM) treatment and values were normalized to vehicle-treated (DMSO) cells. Both box plot graphs show the mean values from the eight HCC cell lines grouped by their specific RAS and RAF status. P values < 0.05 were considered statistically significant and are indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant