Fig. 5.

Yoda1 facilitates tension-induced arm motions. a RMSD for each Piezo1 arm along the full multi-ligand trajectory. b Trajectory snapshots of the Yoda1-bound subunit (arm 3) taken before (4.8 µs) or after (6.9 µs) stretch. c PCA representation of tilt motions. The green arrows indicate the direction of tilt motions along the stretch region of the trajectory. d Time evolution of tilt angle for each arm along the multi-ligand trajectory. e PCA representation of twist motions. The purple arrows indicate the direction of twist motions along the stretch region of the trajectory. f 2-dimensional projection of tilt and twist motions (represented as total C_α motion) colored as function of time during the stretch region of the trajectory. The 0 value in the tilt and twist axes represent the average position along the corresponding PCA motion