Fig. 3. Depletion of HMGA1 and HIPK2 causes a drastic reduction of Surfactant expression levels.

a RNA extracted from lungs of WT and DKO mice at P1 were analyzed by qRT-PCR for Sp-A, Sp-B, and Sp-C expression. The Actin expression level has been used for normalization. n = 3 WT mice and n = 10 DKO mice were analyzed. Data are mean ± SD of a representative experiment performed in triplicate; *P < 0.05, **P < 0.01 (Student’s t test). b Analysis of SP-C protein levels by western blot experiments and relative densitometry from proteins extracted from lungs of n = 3 WT and n = 5 DKO mice. Vinculin was used for normalization. c Immunohistochemistry analysis of SP-C at P1 WT versus DKO lung (100x magnification). One representative experiment is shown