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. 2019 Oct 3;10(10):747. doi: 10.1038/s41419-019-1975-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b H&E staining of WT and DKO thyroid glands of mice at P1 (× 200 magnification). c–j NKX2.1, FOXE1, PAX8, and TG on WT c, e, g, i and DKO d, f, h, j thyroid sections (× 20 magnification). One representative experiment is shown. k RNA extracted from portion of neck containing thyroid gland of WT and DKO mice at P1 was analyzed by qRT-PCR for Pax8, Tg, Tpo, and TSHr expression. n = 3 WT mice and n = 6 DKO mice were analyzed. The Actin expression level has been used for normalization. Data are mean ± SD of a representative experiment performed in triplicate. * indicates significant difference P < 0.05, ***P < 0.001 (Student’s t test)