Fig. 5. LRRC7 enhances mGluR5 signaling in neurite growth.

a EGFP transfected hippocampal neurons were measured at 7 DIV. Mutant neurons showed reduced neurite growth. Student's t-test, ***P < 0.001. b mGluR5 specific antagonist MPEP reduced branch number and length in wild-type neurons, but did not further reduce neurite growth in mutant neurons (two-way ANOVA: branch points, drug: F_(1,63) = 7.99, P = 0.0063; length, drug: F_(1,63) = 8.72, P = 0.0044, Bonferroni post hoc test, *P < 0.05, **P < 0.01, ***P < 0.001). c Addition of CDPPB rescued the neurite growth defect in mutant neurons (two-way ANOVA: branch points, genotype: F_(1,42) = 5.55, P = 0.0232; length, genotype: F_(1,42) = 5.68, P = 0.0218). Bonferroni post hoc test, *P < 0.05, **P < 0.01, n.s., not significant. Number of neurons as indicated in bars. Neurons collected from three independent experiments. +/+, wild-type; m/m, homozygous Lrrc7 mutant