Fig. 5. Poly-PR-induced suppression of hnRNPF/H1 function causes neurotoxicity.

a, b NSC-34 cells were infected with adenovirus encoding LacZ or hnRNPF at an MOI of 200. Cells were also co-infected with adenovirus encoding LacZ or FLAG-PR100 at an MOI of 400. At 48 h after the infection, the cell lysates were subjected to immunoblotting (IB) and dot blotting analysis using indicated antibodies (a). Intensities of immunodetected signals of endogenous hnRNPH1 were densitometrically examined with an ImageJ software (b). The data are presented as means ± SD (N = 3). Statistical analysis was performed by one-way ANOVA followed by the Dunnett’s multi comparisons test. c, d NSC-34 cells were co-transfected with the 5 nM control, hnRNPF, and hnRNPH1 siRNAs. To keep the total concentration of siRNA at 10 nM, an appropriate amount of control siRNA was added for each transfection. At 60 h after the transfection, the cell viability was detected by WST-8 assay (c). The cell lysates were subjected to immunoblotting (IB) analysis using indicated antibodies (d). The data are presented as means ± SD (N = 3). Statistical analysis was performed by one-way ANOVA followed by the Tukey’s multi comparisons test