Fig. 4.

Gnat3^−/− mice develop distinct oral microbiota. a Principal component analysis (PCA) of microbiota recovered from ligatures around molars of WT and Gnat3^−/− mice (n = 10 mice). Each circle represents an individual ligature sample, colored by genotype. b Operational taxonomic unit (OTU)-level correlation network analysis of ligature microbiota from WT and Gnat3^−/− mice (n = 10 mice). The most abundant 50 OTUs from each genotype were analyzed. Each circle represents one OTU, colored by phylum. The size of a circle indicates the abundance of the OTU. Green lines indicate positive correlations between two OTUs; red lines indicate negative correlations. c Average genus-level composition of ligature microbiota from WT and Gnat3^−/− mice (n = 10 mice). d Prevalent genus with significant difference in abundance between WT and Gnat3^−/− mice (n = 10 mice, means ± 95% confidence interval). ***p < 0.01, Wilcoxon rank-sum test. e qPCR quantification of NI1060, a ligature-induced periodontitis-related pathogen. Result of each ligature sample is plotted, with black line indicating means ± SEM (n = 10 mice). ***p < 0.01, Wilcoxon rank-sum test. Source data are provided as a Source Data file