Fig. 3.

Intranasal immunization induces production of anti-PT and anti-FHA IgG in serum. ELISAs were used to compare serological responses from mice immunized through IN or IP routes to mock vaccinated mice. Total IgG serum antibody titers from immunized and challenged mice were quantified against a PT and b FHA at day 3 pc. Serum c IgG1 and d IgG2 antibody titers against B. pertussis were compared to mock vaccinated mice at day 3. Results shown as mean ± SEM, **P < 0.01, ***P < 0.001, ****P < 0.0001 (n = 3–8). P values were determined by one-way ANOVA with Dunnett’s post hoc test compared to mock vaccinated mice