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. 2019 Oct 3;10(10):754. doi: 10.1038/s41419-019-1985-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a) Representative images of immunohistochemistry staining for Phlpp1 (red) in human IVDs with degeneration grade 1–5. Left: Phlpp1 stain in the NP, right: Phlpp1 stain in the AF. Arrows indicate positive cells. Neg.: negative control of Phlpp1 stain with primary antibody being replaced by negative control IgG. b+c) Quantification of staining for Phlpp1 and its correlation with b) degeneration grade and c) age. Expression of Phlpp1 was strongly correlated with the degeneration grade and to a lesser extent with age both in the NP and AF (n = 16)