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. 2019 Oct 3;9:14284. doi: 10.1038/s41598-019-50763-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Examination the gonads of the hybrid embryos: p63 and SSEA-1 immunostaining identify the endogenous PGCs in the gonads of hybrid embryo at day-10 (16H02) and p63 at day-20 (16H07). (A) The SSEA-1 expressing PGCs are red colored on the cell surface. The p63 expressing PGCs are green colored in nucleus. White square shows the cells on the picture (A) (right top). (B) The p63 expressing PGCs are green colored in nucleus. White square shows the cells on the picture (B) (right bottom). White arrows demonstrate two host derived PGCs. For nuclear staining (nucleus) we used Hoechst 33342 staining (blue).