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. 2019 Oct 3;9:14284. doi: 10.1038/s41598-019-50763-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of the endogenous host-derived and injected GFP expressing donor derived PGCs in the left and right gonads of 18.5-day-old hybrid embryo (19H04). (A) CVH expressing PGCs are red colored in the cytoplasm. The donor derived GFP expressing PGCs are green colored. White square shows the cells on the picture (A1–A4). Whyte arrows demonstrate two host derived PGCs. Green arrows indicate two integrated donor derived GFP expressing PGCs. (A1) Confocal merge images of CVH (red), GFP (green) and nuclear stained (blue) PGCs. (A2) Confocal images of CVH (red) stained PGCs. (A3) Confocal images of GFP expressing PGCs. (A4) Confocal images of nuclear stained PGCs. For nuclear staining (nucleus) we used TO-PRO®-3 stain (blue). Scale bars: 100 μm (A), 25 μm (A1–A4).