Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 3;10:4491. doi: 10.1038/s41467-019-12472-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Organoid functionality after hypoxic stress. a Fluorescence views of IC spheroids and IC-hAEC organoids exposed to hypoxia and assessed for viability by a FDA/PI test; green (FDI) and red (PI) signals indicate live and dead cells respectively. Scale bars = 100 μm. b Insulin secretion, expressed as SI, of IC spheroids (magenta columns) and IC-hAEC organoids (blue columns) under normoxic and hypoxic conditions, *p < 0.03 and after hypoxic exposure, *p < 0.02, two-way ANOVA with Sidak’s multiple comparisons test, n = 5. c Insulin mRNA expressed by IC and IC + hAEC spheroids cultured under hypoxic conditions; insulin mRNA was analyzed by qPCR, and data presented as arbitrary units (AU) after normalization to housekeeping genes, ***p < 0.0003, unpaired Student’s t test, n = 3. d, e HIF-1α nuclear localization visualized by immunostaining and its upregulated expression in IC-hAEC spheroids. ****p < 0.0001, unpaired Student’s t test, n = 6. Scale bar = 50 μm. f Casp3, Casp8, Casp9, and Bcl2 mRNAs expressed by IC spheroids spheroids (magenta columns) and IC + hAEC organoids (blue columns) cultured under hypoxic conditions; data presented as arbitrary units (AU) after normalization to housekeeping genes. ***p > 0.0003, **p < 0.0075, *p < 0.02, unpaired Student’s t test, n = 3. All data shown are means ± SEM