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. 2019 Sep 27;10:1200. doi: 10.3389/fpls.2019.01200

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Copyright © 2019 Pérez-Pérez, El-Tantawy, Solís, Risueño and Testillano

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Positive and negative control experiments of IAA immunofluorescence on growing root tips of barley. Confocal microscopy images of IAA immunofluorescence (green signal). (A–C) Positive control of IAA immunofluorescence on root tips. (D–F) Negative control of IAA immunofluorescence on roots using anti-IAA antibodies preblocked with IAA (immunodepletion). (A, D) Differential interference contrast (DIC). (B, E) DAPI staining of nuclei in blue. (C, F) IAA immunofluorescence in green. Bars represent 100 µm.