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. 2019 Sep 27;10:1200. doi: 10.3389/fpls.2019.01200

Figure 8.

Figure 8

Immunolocalization of IAA in microspore embryogenesis cultures treated with kynurenine (inhibitor of auxin biosynthesis) and NPA (inhibitor of polar auxin transport). Confocal microscopy merged images of IAA immunofluorescence (green signal) and DAPI staining for nuclei (blue signal). (A) Proembryos from 40 µM kynurenine-treated cultures, after 4 days in culture. (B) Proembryos from 3 µM NPA-treated cultures, after 4 days in culture. (C) Developing embryo from 3 µM NPA-treated cultures, after 30 days in culture. Inset: Details at higher magnification of an embryo region from 3 µM NPA-treated cultures. Bars represent (A, B, D) 20 µm, (C, inset) 200 µm.