Fig. 6. NF-κBp65 deficiency in gastric myeloid cells attenuated Fas-mediated epithelial apoptosis in PHG.
a Gastric mucosal apoptosis was depressed in PVL-treated p65ΔM/ΔM (myeloid cells specific NF-κBp65 deletion) mice compared with p65f/f (floxed p65) mice (TUNEL staining, × 400, n = 6 per group). b Double staining of cytokeratin (green) and TUNEL (apoptotic cells, red) indicated that gastric apoptotic cells mainly located in the epithelial cells of the gastric mucosa from PVL mice (upper, × 400; lower panel, × 800, n = 6 per group). c The apoptotic index from TUNEL staining was presented. n = 6 in each group, values are presented as mean ± SEM. *P < 0.05 versus SO (sham operation) mice, #P < 0.05 versus PVL-treated p65f/f mice. d Western blotting analysis demonstrated that deletion of NF-κBp65 in myeloid cells blocked Fas, cleaved caspase-3 levels and NF-κBp65 phosphorylation in the gastric epithelial cells from PVL-treated mice. β-actin was used as the loading control. n = 6 per group