Figure 5.

DSS-induced colitis increased the numbers of activated macrophages in WT mice. To assess macrophage activation, paraffin sections from the various treatment groups were stained with Iba-1, a protein upregulated during activation of macrophages and possibly neutrophils (black arrows). Representative sections harvested from vehicle-treated (a) WT or (b) Tmem173^gt mice, or from DSS-treated (c) WT or (d) Tmem173^gt mice or from DSS treated (e) WT or (f) Tmem173^gt mice that were co-treated with DMXAA (5 mg/kg). Although there were no discernable differences in macrophage activation between the untreated WT and Tmem173^gt groups of mice, Iba-1 staining of colonic tissues from the DSS-treated WT and DSS-treated WT mice co-treated with DMXAA looked to be increased when compared with similarly-treated Tmem173^gt mice. All sections were harvested on day 7 from mice treated with 3% DSS. Anti-Iba-1 immuno-histochemical stain. Sections imaged using a 20x objective.