Fig. 7.
The ionic anchor points are structurally and functionally conserved in TRIM 5. a Clustal omega multiple sequence alignment of TRIM proteins. Ionic anchor points are highlighted in red. The sequences of TRIM21 and TRIM5 RING domains are highly similar (sequence identity: 47.3, sequence similarity: 63.4 in pairwise sequence alignment; Supplementary Fig. 6). b Close-up of the tri-ionic motif in the structural model of TRIM5-RING Ube2N~Ub complex based on superposition of the T5-R Ube2N (4TKP)22 and our T21-R Ube2N~Ub structure (the full model is shown in Supplementary Fig. 11b). c Catalysis of unanchored ubiquitin chains by Ube2N/Ube2V2 of T5-R mutants. d Catalysis of ubiquitin discharge from ubiquitin conjugated Ube2N/Ube2V2 of T5-R mutants. Source data are provided as a Source Data file