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. Author manuscript; available in PMC: 2020 Mar 18.
Published in final edited form as: Nature. 2019 Sep 18;574(7776):57–62. doi: 10.1038/s41586-019-1570-z

Extended Data Figure 1 |. Generation of FPR1−/− U937 cells using CRISPR-Cas9 and genetic complementation.

Extended Data Figure 1 |

a, Sequencing results for alleles cloned from CRISPR-Cas9 derived FPR1−/− U937 macrophages. b, Immunoblot analysis for the production of FPR1, FPR2 and FPR3 in U937 macrophages, CRISPR-Cas9 derived FPR1−/− cells and FPR1−/− cells transfected with pFPR1. Numbers to the left of blots indicate migration of molecular weight markers. One of three repeats is shown (a, b).