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. Author manuscript; available in PMC: 2020 Mar 18.
Published in final edited form as: Nature. 2019 Sep 18;574(7776):57–62. doi: 10.1038/s41586-019-1570-z

Extended Data Figure 2 |. Contribution of CCR5 to Yersinia spp. intoxication and injection by the T3SS.

Extended Data Figure 2 |

a, Sequencing results for the alleles of two CCR5−/− U937 isolates obtained using CRISPR-Cas9 mutagenesis. b, Cell survival following incubation with strains Y. pestis KIM D27 and POO1 was measured using the Trypan blue exclusion assay; error bars represent the s.e.m. (n = 4 biological replicates). T3SS injection into U937, FPR1−/−, and CCR5−/− by Y. pestis KIM D27 (c) and Y. pseudotuberculosis YPIII carrying pMM83 (YopM-Bla) (d); error bars represent the s.e.m. (n = 3 biological replicates). One of three repeats is shown (b-d). Statistical analysis was performed using one-way ANOVA with Bonferroni Correction: ***, P <0.001; **, P <0.01.