Figure 4.

Inhibition of AP‐1 reduced microglial production of IL‐6 and TNF‐α in ICH. A, Microglia expressing IL‐6 and TNF‐α were analyzed by flow cytometry. Microglia were isolated from autologous blood‐induced ICH mice who received SR11302 (red) or vehicle (blue) at 6 h after ICH. B, Quantification of microglia expressing IL‐6 and TNF‐α isolated from the indicated groups of ICH mice. n = 6 mice per group. C, The levels of IL‐6 and TNF‐α in cultured microglia after exposure to thrombin (2 U/mL) + vehicle or thrombin (2 U/mL) + SR11302 (10 μmol/L). Microglia were incubated with thrombin and vehicle or SR11302 at 37°C in an incubator with 5% CO₂ and an atmospheric O₂ concentration for 24 h, and their expression of IL‐6 and TNF‐α was analyzed by flow cytometry. n = 3 per group. Mean ± SEM. **P < .01