Table 1.
Primer sequences used for real‐time polymerase chain reaction
Gene | Forward primer | Reverse primer |
---|---|---|
GAPDH | 5′‐GGAAGCTGTGGCGTGATGGC‐3′ | 5′‐TTCTCCAGGCGGCAGGTCAG‐3′ |
iNOS | 5′‐GCTGAAGGCTCTCCACCTCCTC‐3′ | 5′‐GGTATCTTGTCATCGCTGTCATCTCC‐3′ |
TNF‐a | 5′‐GCACTGAGAGCATGATCCGAGAC‐3′ | 5′‐CGACCAGGAGGAAGGAGAAGAGG‐3′ |
IL‐1β | 5′‐TACAGGCTCGTGCAGGACTCAG‐3′ | 5′‐GGTGGTGCGGCTGGATTGC‐3′ |
TGF‐B1 | 5′‐AACCTACCCGACTGGTATC‐3′ | 5′‐CACAGCCGGACCTTTAAC‐3′ |
TGF‐BR2 | 5′‐TACCACGGATTTGTTCTCGAT‐3′ | 5′‐ACCCTTTCCTCTCCTTCATAA‐3′ |
BMP2 | 5′‐CAACAGAGGCAATGGAGC‐3′ | 5′‐TTGGCAGATGGCCTTGTAG‐3′ |
BMP7 | 5′‐GAGATGTTCGAGGCACAC‐3′ | 5′‐GTCTCATTCACTCAGCGGA‐3′ |
MYC | 5′‐AACGTCAGCTTCACCAAC‐3′ | 5′‐AGAAATAAGGCTGCACCG‐3′ |
DUSP2 | 5′‐CTGGTTCCAGGAGGCTATC‐3′ | 5′‐CCTGCCTGGCAATGTACTA‐3′ |
PTPN7 | 5′‐CCTCTGCTTCCTCCAATG‐3′ | 5′‐GGTCAGCCACTAGCTTCAA‐3′ |
NFATC1 | 5′‐TGGTGGTGGACTCATATTCATC‐3′ | 5′‐GAAGAAGCTGAGAAGCTAAAGG‐3′ |
MAP4K1 | 5′‐TTTCATCCTGAACCGAAATGAC‐3′ | 5′‐GAGAGAGACATGAGGACATTG‐3′ |