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. Author manuscript; available in PMC: 2019 Oct 4.
Published in final edited form as: Sci Transl Med. 2019 Aug 14;11(505):eaav6278. doi: 10.1126/scitranslmed.aav6278

Fig. 3. Loss of Ghsr replicates AD-like phenotypes.

Fig. 3.

(A) Analysis of synaptic density in CA1 regions from 4- and 9-month-old mice. **P < 0.001 and ***P < 0.001 nonTg versus other groups at the same age, one-way ANOVA followed by Bonferroni post hoc analysis. Four-month-old mice: nonTg, n = 4; 5×FAD, n = 7; Ghsr null mice, n = 5; and Ghsr null/5×FAD, n = 4. Nine-month-old mice: nonTg, n = 4, 5×FAD, n = 4, Ghsr null mice, n = 4, and Ghsr null/5×FAD, n = 3. (B) Representative 3D reconstructed images of synapse staining. Vesicular glutamate transporter 1 (vGLUT1, blue) and postsynaptic density 95 (PSD95, red) were used to visualize pre- and postsynaptic terminals, respectively. The overlapped staining of vGLUT1 and PSD95 indicates synapses. (C) Time course of LTP and representative fEPSP responses during the baseline period (black trace) and 30 s after theta burst simulation (red trace) in four groups of mice at 9 months old. *P < 0.05 and ***P < 0.001 nonTg versus other groups, one-way ANOVA followed by Bonferroni post hoc analysis. nonTg, n = 5; 5×FAD, n = 4; Ghsr null, n = 5; and Ghsr null/5×FAD, n = 5. (D to I) Spatial navigation of four groups of mice in the Morris water maze test. (D and G) Spatial learning of four groups of mice at 4 (D) and 9 (G) months old. *P < 0.05, **P < 0.01, and ***P < 0.001, nonTg versus other groups on the same day, one-way ANOVA followed by Bonferroni post hoc analysis. (E and H) Spatial reference memory of different groups of mice at 4 (E) and 9 (H) months of age. *P < 0.05, one-way ANOVA followed by Bonferroni post hoc analysis. (F and I) Swimming speed of four groups of mice at 4 (F) and 9 (I) months old. Four-month-old mice: nonTg, n = 7; 5×FAD, n = 9; Ghsr null mice, n = 10; and Ghsr null/5×FAD, n = 6. Nine-month-old mice: nonTg, n = 8; 5×FAD, n = 11; Ghsr null mice, n = 5; and Ghsr null/5×FAD, n = 8.