Figure 2.

The effect of starvation on cell proliferation. (A) Light micrographs of the testes of fed and starved male prawns showing anti-Ki67 staining of dividing cells in the seminiferous tubules. The numbers of anti-Ki67-stained germ cells at day 8 were higher in the crescentic areas of the tubules of fed prawn (a–c) compared to those of starved prawns (d–f) whose tubules contain smaller crescentic areas with fewer number of anti-Ki67-stained germ cells. (B) Comparison of the numbers of Ki67-positive germ cells between fed and starved groups at day 8. Asterisk indicates significant difference (p < 0.05) between the two groups (n = 5).