Effect of local Psm infection on systemic expression of WRKY6, WRKY29, and WRKY53 and SAR. A, Experimental setup for analyzing gene expression (RT-qPCR and RNA-seq) and SAR. B to D, Expression of WRKY6, WRKY29, and WRKY53 in challenged systemic leaves of Local mock (− Local Psm) and Local Psm (+) plants at 2.5 h after the systemic challenge and at various days after the initial treatment (Local mock or Local Psm). dpi, days post initial treatment. Error bars represent the SD (n = 6); ***P ≤ 0.001; **P ≤ 0.01. E, Bacterial titer in systemic leaves of Local mock (− Local Psm) and Local Psm (+) plants at 0 h (d0) and 72 h (d3) after inoculation and at various days after the initial treatment (Local mock or Local Psm). cfu, colony-forming units. Error bars represent the SD (n = 3); **P ≤ 0.01.