RINRKs encode LRR receptor-like kinases. A, RINRK1 and RINRK2 protein structure. RINRKs have an N-terminal signal peptide (SP), three LRR domains, a transmembrane (TM) domain, and a C-terminal RLK domain. The sites of the RINRK1-1 and RINRK2-1 mutations are shown. B to F, Confocal microscopy of root hairs of wild type (B), rinrk1-1 (C), rinrk2-1 (D), and rinrk1 rinrk2 (E and F) 7 d after inoculation with M. loti R7A expressing GFP. Bar = 20 μm. G, Mean number of infection events [infection foci (IF), infection thread (IT)]. H, mean number of nodules formed on wild-type, rinrk1-1, rinrk2-1, and rinrk1 rinrk2 mutants. The infection events were analyzed 7 and 14 d postinoculation (dpi), and the nodule numbers were scored 2, 3, and 5 wpi (weeks postinoculation; ± se, n > 15). *P < 0.05, **P < 0.01 between wild type and mutant at the same time point.