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. 2019 Sep 27;10:1252. doi: 10.3389/fphys.2019.01252

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Copyright © 2019 Tyganov, Mochalova, Belova, Sharlo, Rozhkov, Vilchinskaya, Paramonova, Mirzoev and Shenkman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphorylation status of the signaling proteins in the rat soleus muscle after hindlimb suspension and plantar mechanical stimulation. Quantification of total IRS-1/GAPDH ratio after 1-day HS (A) and 3-day HS (B). Quantification of phospho-eEF2/total eEF2 ratio after 1-day HS (C) and 3-day HS (D). Quantification of phospho-FOXO3/total FOXO3 ratio after 1-day HS (E) and 3-day HS (F). Quantification of phospho-AKT/total AKT after 1-day HS (G) and 3-day HS (H). Representative immunoblots for the studied proteins in the rat soleus muscle (I). C, control group; 1HS, 1-day hindlimb suspension; 3HS, 3-day hindlimb suspension; PMS, 1- or 3-day HS plus plantar mechanical stimulation. All data expressed relative (%) to control. ^*Significant difference vs. C, p < 0.05; ^#significant difference vs. HS. All values are means ± SE, n = 7/group.