Figure EV1. Rab35 localises to the ciliary membrane.

• A, B
  Super‐resolution (FV‐OSR) imaging of hTERT‐RPE1 stably expressing GFP‐RAB35 after 24 h serum starvation and staining for GFP and acetylated tubulin (acetyl. tub.) (A) or GFP, ARL13B and polyglutamylated tubulin (polyglu. tub.). Scale bars, 1 μm.
• C
  Representative images of hTERT‐RPE1 cells stably expressing GFP‐RAB35 after 24 h serum starvation and staining for GFP, ARL13B, polyglutamylated tubulin (polyglu. tub.) and DNA. Higher magnification images of the cilia region shown to the bottom. Scale bars, 10 μm. Graph to the right shows quantification of cilia with GFP‐RAB35 localisation to the proximal region of the cilium marked with polyglutamylated tubulin staining (ARL13B > GFP‐RAB35 = polyglu. tub.) and cilia with GFP‐RAB35 localisation along the full length of the cilium marked with ARL13B staining (ARL13B = GFP‐RAB35 > polyglu. tub.). Note that in ˜15% of cilia all three markers stained the same cilia region (ARL13B = GFP‐RAB35 = polyglu. tub.). Data are mean ± SEM of three independent experiments (n ≥ 30 cilia per experimental condition).