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. 2019 Aug 27;20(10):e48019. doi: 10.15252/embr.201948019

Figure 1. Lack of H3K27me3 and H2AK119ub enrichment over the X chromosome in the absence of Xist repeats B and C.

Figure 1

  1. Schematic representation of the novel Xist‐TetOP mutants generated by CRISPR/Cas9 genome editing in J1 XY ESCs; the different repeats are highlighted in color boxes; Dif.—differentiation; DOX—doxycycline.
  2. Representative images of combined IF for H3K27me3 or H2AK119ub (green) with RNA FISH for Xist (red) in Xist‐TetOP lines (for clone 1 of each mutant type) at day 2 of differentiation in DOX conditions; blue—DAPI staining; scale bar: 10 μm.
  3. Graph represents the mean % + SEM of Xist‐coated X chromosomes enriched for H3K27me3 or H2AK119ub in the different Xist‐TetOP mutants (for clone 1 of each mutant type) from 2 to 4 independent experiments; a minimum of 50 Xist‐coated X chromosomes were counted per experiment; only P‐values corresponding to significant differences from unpaired Student's t‐test comparing mutants to Xist FL are indicated as * (P‐value < 0.05).

Source data are available online for this figure.