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. 2019 Aug 27;20(10):e48019. doi: 10.15252/embr.201948019

Figure EV3. Quality check of ChIRP procedure in Xist FL and Xist ΔB+C cells.

Figure EV3

  1. RT–qPCR with three primer pairs along Xist to evaluate RNA retrieval after ChIRP procedure for Xist FL (in noDOX and DOX conditions) and Xist ΔB+C (DOX) at day 3 of differentiation.
  2. Table showing the percentage of cells exhibiting a Xist‐coated X chromosome for Xist FL (both noDOX and DOX) and Xist ΔB+C (DOX) as determined by Xist RNA FISH used for ChIRP‐MS; a minimum of 500 cells were counted.
  3. Blot visualized with Coomassie blue staining showing the band pattern of proteins displayed by Xist FL (both noDOX and DOX) and Xist ΔB+C (DOX) after ChIRP.