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. 2019 Aug 14;20(10):e47788. doi: 10.15252/embr.201947788

Figure EV2. Binding of DFL to CXCL12 assessed by NMR and MST .

Figure EV2

  1. Superposition of 1H‐15N HSQC spectra of CXCL12 (˜0.1 mM, pH 6, phosphate buffer) without (black) and with 10‐fold excess of DFL (orange).
  2. Weighted average of Q48 and V49 amide 1H and 15N chemical shift changes in the presence of increasing concentration of DFL. Nonlinear curve fitting yields K d = 802 ± 102 μM.
  3. Dose–response curve of CXCL12‐DFL interaction followed by MST. DFL concentrations ranged from 5 mM to 153 nM on 50 nM labeled 6His‐CXCL12. The K d is 2.6 ± 1.2 mM (n = 3, error bars correspond to SD). Saturation of the curve could not be reached because of solubility issues for DFL stock solutions > 10 mM. (Fnorm = normalized fluorescence).

Source data are available online for this figure.