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. 2019 Aug 14;20(10):e47788. doi: 10.15252/embr.201947788

Figure 3. Effect of DFL on the HMGB1/CXCL12 heterocomplex.

Figure 3

  • A, B
    1H‐15N HSQC HMGB1 (0.1 mM) spectrum (A) without (black) and (B) with 0.2 mM CXCL12.
  • C
    MST measurements of CXCL12 titrated into 50 nM fluorescently labeled HMGB1; the signal increases from ˜930 (free) to ˜950 a.u. (bound) yielding an apparent K d of 4 ± 0.4 μM. n = 3; data represent avg ± SD.
  • D, E
    H‐15N HSQC of HMGB1 (0.1 mM) with CXCL12 (0.2 mM) upon addition of (D) 0.2 mM and (E) 1 mM DFL.
  • F
    MST measurements of the heterocomplex (preformed using 50 nM HMGB1 and 50 μM CXCL12) in the presence of increasing concentrations of DFL. Decreasing MST signal (from ˜950 to ˜930 a.u.) upon DFL titration indicates that CXCL12 has been displaced from HMGB1. n = 3; data represent avg ± SD.

Source data are available online for this figure.