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. 2019 Aug 14;20(10):e47788. doi: 10.15252/embr.201947788

Figure EV4. DFL does not affect the cytokine‐inducing activities of disulfide HMGB1 on human macrophages.

Figure EV4

  • A–C
    Human macrophages were obtained as described 6 and activated or not for 3 h with 3 μg/ml disulfide HMGB1 (˜100 nM) or 10 ng/ml LPS, in the presence of the indicated concentrations of DFL. The bars represent the avg ± SD (n = 3 biological replicates). In all cases, the mRNA levels (of IL6, IL8, TNF) in cells exposed to disulfide HMGB1 (ds‐HMGB1) are not different in the presence of increasing concentrations of DFL (one‐way ANOVA plus post‐tests). This experiment was performed twice with macrophages generated from different donors.

Source data are available online for this figure.